Fig 1: Hyaluronidase PH20 expression and stroma disruption effects of VCN-01 in patients. (A) PH20 serum levels as determined by ELISA at the indicated time points in patients treated with VCN-01 in combination with nab-paclitaxel plus gemcitabine (GA). Each filled circle represents a serum sample per patient. Analyzed patients were injected at a dose of 1×1011 vp of VCN-01 (six patients). One-way ANOVA (Tukey’s multiple comparisons test) was used to evaluate multiple comparisons between groups. PH20 expression in serum samples at different time points is expressed as pg/mL minus background levels detected for each sample at day 0 and represented in box and whiskers graphs. (B) Changes in strain ratio measured by endoscopic ultrasound (EUS) elastography in five patients prior to cycles 1, 2 and 3. Each line represents an individual patient. ANOVA, analysis of variance.